apugalde
mikelove
/ deseq2_curves.R
Created
June 15, 2023 06:17
Drawing DESeq2 fitted spline curves on top of scaled counts
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| library(splines) | |
| library(DESeq2) | |
| # make some demo data | |
| dds <- makeExampleDESeqDataSet(n=100, m=40) | |
| dds$condition <- sort(runif(40)) | |
| # make one gene where expression has a curve shape (just for demo) | |
| s_shape <- round(500 * sin(dds$condition*2*pi) + 1000 + rnorm(40,0,50)) | |
| mode(s_shape) <- "integer" | |
| counts(dds)[1,] <- s_shape |