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| Hello Python Discord |
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| [tool.poetry] | |
| name = "issue" | |
| version = "0.1.0" | |
| description = "" | |
| authors = ["Fin Swimmer"] | |
| exclude = ["issue/exclude_me.py"] | |
| [tool.poetry.dependencies] | |
| python = "^3.6" |
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| rule target: | |
| input: | |
| bam = "SRR7526729_realigned.bam", | |
| index = "SRR7526729_realigned.bam.bai" | |
| rule umi_extract: | |
| input: | |
| R1 = "../{sample}_1.fastq.gz", | |
| R2 = "../{sample}_2.fastq.gz" | |
| output: |
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| kmer | count | |
|---|---|---|
| AAA | 72422544 | |
| AAC | 43498931 | |
| AAG | 58395299 | |
| AAT | 72538809 | |
| ACA | 54592537 | |
| ACC | 33744272 | |
| ACG | 7570315 | |
| ACT | 47439444 | |
| AGA | 60011989 |
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| #!/usr/bin/env python | |
| # -*- coding: utf-8 -*- | |
| from __future__ import print_function | |
| import sys | |
| from argparse import ArgumentParser | |
| def main(): | |
| args = get_args() |
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| configfile: | |
| "config.json" | |
| SAMPLES, = glob_wildcards(config['data']+"/{id}_L001_R1_001.fastq.gz") | |
| rule move: | |
| input: | |
| bam = expand("{sample}.final.bam", sample=SAMPLES), | |
| bai = expand("{sample}.final.bai", sample=SAMPLES) | |
| params: |
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| configfile: | |
| "config.json" | |
| SAMPLES, = glob_wildcards(config['data']+"/{id}_L001_R1_001.fastq.gz") | |
| rule move: | |
| input: | |
| bam = expand("{sample}.final.bam", sample=SAMPLES), | |
| bai = expand("{sample}.final.bai", sample=SAMPLES) | |
| params: |
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| from Bio import AlignIO | |
| def column(alignment): | |
| '''Generator for getting list of characters in each column in the alignment''' | |
| for i in range(alignment.get_alignment_length()): | |
| c = [] | |
| for record in alignment: | |
| c.append(record.seq[i]) |
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| ENSPFO Poecilia formosa (Amazon molly) | |
| ENSJJA Jaculus jaculus (Lesser Egyptian jerboa) | |
| ENSPCO Propithecus coquereli (Coquerel's sifaka) | |
| ENSNGA Nannospalax galili (Upper Galilee mountains blind mole rat) | |
| ENSMFA Macaca fascicularis (Crab-eating macaque) | |
| ENSMIC Microcebus murinus (Mouse Lemur) | |
| MGP_CAROLIEiJ_ Mus caroli (Ryukyu mouse) | |
| ENSFAL Ficedula albicollis (Flycatcher) | |
| ENSCLA Chinchilla lanigera (Long-tailed chinchilla) | |
| ENSPEM Peromyscus maniculatus bairdii (Northern American deer mouse) |
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| import sys | |
| from Bio import SeqIO | |
| def mismatch(seq1, seq2): | |
| index = [] | |
| for i, (s1, s2) in enumerate(zip(seq1, seq2)): | |
| if s1 != s2: | |
| index.append(i) |
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